Abstract: Brucellosis, caused by Brucella, was a infectious diseases of livestock and human chronic bacteria widely distributed in the world with the abortion and fever as characteristics and seriously threated to the life healthy of human and many kinds of animal. Brucella could infected human, many kinds of livestock and wild animals and resulted in enormous economic loss and serious public health problem. Thus, the testing work of Brucella was the important part to prevent its occurrence. The author summarized the detection technology of Brucella such as immunology ELISA, colloidal gold, PCR. Those technologies all could detect the Brucclla accurately, quickly, and specifically.