Abstract: The purpose was to discuss the bone nutritional condition of grazing sheep around Qinghai Lake. The contents of hydroxyproline, calcium and phosphorus and activities of alkaline phosphatase in serum of 55 2-3-year old grazing sheep from 4 counties in the region around Qinghai Lake were determined by dimethylaminobenzaldehyde colorimetry, malachite green-molybdic acid colorimetry, MTB colorimetry and disodium phenyl phosphate method. The results showed that the contents of hydroxyproline in serum of determined grazing sheep were （ 15.359±1.655） μmol/L, their activities of alkaline phosphatase in serum were （2.797±0.741） nmol/（L.s）, their contents ef calcium and phosphorus in serum were （2.443±0.291 ） and （1.210±0.390）mmol/L resp. and their contents of inorganic phosphorus in serum were significantly lower than normal value （P〈0.05）. The 55 determined grazing sheep were at the nutrition level of low phosphorus.