Abstract: In order to study the effects of in vitro preservation and long-distance transportation of fresh embryo for more than five hours on the transfer efficiency of embryo,the sheep of donor and receptor were fed separately.After the fresh embryos from donors were transported for more than 300 km,the embryos were transplanted in recipient sheep.The results showed that the estrus rate of donor sheep and recipient sheep were 100% and 97.9% respectively after estrus synchronization treatment.The number of embryos obtained from oviduct of a donor was 13.5.The lambing rate of the receptor was 79.2%,slightly higher than that from direct transfer.In conclusion,long-distance transportation and in vitro preservation for over five hours had no significant effect on the transfer efficiency of embryos when recipient sheep had good nutritional status,appropriate management and in vitro preservation method.