Abstract: The minimal inhibitory concentration of eight kinds of botanical natural bacteriostats（such as Syringa vulgaris） to Escherichia coli,Staphylococcus aureus,Candida albicans,Mucor racemose was determined by using tube double dilution method and bacteriostatic test method. The results showed that the inhibitory concentration of Folium Isatidis was the lowest among eight kinds of botanical natural bacteriostats（such as S. vulgaris） ,so Folium Isatidis was the preservative with the best bacteriostatic effect.