Abstract: The artificial propagation of loach has been an important method for loach intensive culture,among them artificial induced spawning is also the core part of artificial propagation.Different ratios of oxytocin was used for the artificial induced spawning of loach,and then the number of ovum obtained from natural fertilization was recorded,and drug cost was compared.Results showed that the higher oxytocin was,the better spawning effect was.Under the same drug cost,the number of ovum produced by mixed induced spawning was higher than that of single induced spawning,among them 50 IU/0.2 mL HCG＋0.25 μg/0.2 mL LRH-A2＋0.2 mg/0.2 mL DOM had the best effect.