Abstract: In order to study the relationship between cows hoof disease and milk yield, 15-day daily mean milk yield of 15 infected cows from some national dairy farm in Ningxia Province were investigated, meanwhile through five healthy cows were taken as control, the effects of hoof disease on cows＇ milk yield were compared. Then, the 15 infected cows were carried out specific treatment, after that the daily mean milk yield of 15 infected cows was determined every five days, continuously for six times, and the change law of milk yield was analyzed finally. Results showed that hoof disease could cause a disease of milk yield, furthermore the more serious hoof disease was, the less milk yield of cow was; after treatment, the daily mean milk yield of 15 infected cows with different degrees of hoof disease were improved.