Abstract: The use of microbial fermentation system to take erythritol shows prospects, and the development of its unique attracts the attention of many domestic research institutes and manufacturers, but the high production cost has limited the development of erythritol industry, so reducing the the cost of production of erythritol is the fundamental way to promote the industrial development of erythritol industry. In this experiment, the optimal medium ratio of one erythritol high-yielding strain Candida lipolytica Screened by Shandong Longlive Bio-technology Co., Ltd. Laboratory was taken as material was studied through single factor and orthogonal experiments to improve yield and reduce costs in the actual production.