Abstract: Triticale was the first allopolyploidy that was formed by crossbreeding wheat and rye, which inherited the high yield and high quality of wheat and strong stress resistance. The expression situations of starch synthesis genes and starch content in triticale in different development stage and under different salinity stress were determined by the real-time PCR technology,so as to further understand the effects of salinity stress on starch synthetase gene and starch content. The results showed that the expression of different starch synthetase gene under different degree of salinity stress had different degree of variation and the changes of starch content had a direct correlation with the expression of starch synthetase gene. The study results showed that the salinity stress could change the composition and properties of starch.