Animal Husbandry and Feed Science ›› 2014, Vol. 35 ›› Issue (4): 23-23.doi: 10.12160/j.issn.1672-5190.2014.04.011

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Preliminary Research on the Quality Standards Chinese Herbal Medicine Feed Additive Compound Preparation "Lianteng"

TAO Jun-yu, WEI Si-lian, LI Jing-ling, HU Ting-jun (College of Animal Science and Technology, Guangxi University, Nanning 530005, China)   

  • Online:2014-04-20 Published:2014-04-20

Abstract: The compound preparation "Lianteng" was composed of Chinese herbal medicine Forsythia suspensa Vahl and Caulis spatholobi so as to establish the quality standards of compound preparation "Lianteng". Phillyrin and formononetin in the Chinese medicine preparation were identified by using thin-layer chromatography. And its content was determined by using highperformance liquid chromatography. The chromatographic conditions were as follows: phenomenex luna C 18 (250 mm×4.60 mm, 5 μm)chromatographic column, column temperature of 25 ℃, eluting with methyl cyanide-0.3% phosphoric acid solution as mobile phase, flow rate of I mL/min, the detection wavelength of 254 nm and 277 nm. The results showed that phillyrin and formononetin were separated by using thin-layer chromatography with clear spots. There was a good linear relation of phillyrin at the sample amount of 25-100 μg (R2= 0.999 5 )and the average recovery was 96.40% (n=6). There was a good linear relation of formononetin at the sample amount of 12-120 μg (R2=0.999 8)and the average recovery was 95.13% (n=6).The methods of HPLC and TLC were rapid, acute and repeatable, which was suitable for the quality control of compound preparation "Lianteng".

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