Abstract: A case of piglet diarrhea caused by pseudorabies virus was diagnosed and analyzed in the laboratory. The results showed that a band with the size of 270 bp was shown in the detection sample of PRV. But the target DNA segment was not seen in the detection samples of PEDV, TGEV, CSFV, PRRSV and PCV-2, being negative. The detection results of bacterial pathogen showed that no bacterium was grown. The results of gE antibody detection of female swine herd showed that the results of PRV gE antibody in 33 serum samples were positive and the positive rate reached 91.7%.