Abstract: The immune antibody of O-type foot-and-mouth disease in 60 serum samples were detected by using two test methods of O-type foot-and-mouth disease indirect hemagglutination antibody and O-type foot-and-mouth disease antibody liquid-phase blocking ELISA. The results showed that the qualification rate of 60 serum samples in O-type foot-and-mouth disease indirect hemagglutination assay was 93.3% and that in O-type foot-and-mouth disease antibody liquid-phase blocking ELISA was 73.3%. The qualification rate in O-type foot-and-mouth disease indirect hemagglutination assay was obviously higher than that in O-type foot-and-mouth disease antibody liquid-phase blocking ELISA （higher 20% ）. The whole coincidence rate of two methods was 66.7%, that of less than 25（less than 26） was 28.6% and that of ≥25（ ≥26）was 82.1%. The whole immune effects of two detection methods were both better, and the average qualification rate was greatly higher than the specified value （70%） of Ministry of Agriculture.