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Animal Husbandry and Feed Science ›› 2014, Vol. 35 ›› Issue (10): 7-7.doi: 10.12160/j.issn.1672-5190.2014.10.004

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Amplification of Ghrelin Gene in Oviduct Epithelial Cells of Sheep by RT-PCR

GAO Tian, WEN Shi-yong, QIAN Ying-hong, CONG Shan, LI Qi, SONG Jin, CAO Gui-fang （ 1.College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China; 2.Inner Mongolia Medical University,Hohhot 010110,China;3.Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031, China）   

  • Online:2014-10-20 Published:2014-10-20

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Abstract: Total RNA was extracted from oviduct epithelial cells of sheep,primers were designed by Primer 5.0 software,and Ghrelin gene was amplified by RT-PCR. The results showed that 200 bp of aim gene was obtained successfully by RT-PCR, which indicated that RT-PCR technique was an efficient mean to detect expression of Ghrelin gene in oviduct epithelial cells of sheep. The results lay the basis for studying expression change of Ghrelin gene in oviduct tissues of sheep under different hormone levels and further in vitro test.

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