Abstract: Rotavirus（RV） is an important gastrointestinal pathogen which can cause severe diarrhea in young animals and infants, and it causes a large number of death people and animals as well as great economic loss. WHO called for the development of RV vaccine as one of the highest priority to prevent RV infection. The research advances on molecular biology of RV were summarized in order to lay the basis for developing related gene of porcine rotavirus.