Abstract: A high-performance liquid chromatography （HPLC）-photodiode array detector （DAD） method was established for determination of paracetamol, dipyrone, phenazone, naproxen, aminopyrine illegally added into veterinary antibiotics injection and soluble powder. Samples were extracted under uhrasonication with methanol. A CAPCELL PAK MG Cis analytical column （4.6 mm ×250 mm, 5 μm） was used for the column temperature was at 30 ℃ and the detection wavelength was set at 229 nm. Acetonitrile-phosphate buffer was used as the mobile phase under the program of gradient elution at a flow rate of 1.0 mL/min. The calibration curves of paracetamol, dipyrone, phenazone, naproxen and aminopyrine showed good linearity in the ranges of 5.04-100.88, 12.04-240.72, 5.04-100.72, 2.02-40.40 and 5.02-100.32 μg/mL. The average recoveries （n=5） were 97.9%- 102.8%, 91.9%-98.8%, 96.2%-101.1%, 94.1%-102.3% and 96.0%-102.8%, and RSDs were 1.0%-3.l%, 0.9%-6.4%, 1.7%-4.2%, 1.4%-3.9% and 0.9%-4.8%, respectively. The methodology validation showed that this method was simple, accurate and reliable, so it was appropriate for the determination of the antipyretic analgesic drugs in many kinds of veterinary antibiotics.