Thermopsis lanceolate contains many kinds of alkaloids, which are not only the toxic components of poisoned animal, but also have expectorant and antitussive effect. The alkaloids of T. lanceolate have been reported widely, but little is known about the extraction and separation of alkaloids from the plant. Two crystallizations were purified from T. lanceolate by column chromatography and were ultimately identified as thermopsine and tetrahydrodeoxoargentamin by using TLC, GC-MS and IR spectrometry. The results showed that the method was a simple and reliable method for separation and purification of alkaloid from T. lanceolate, thus provide the safeguard for the follow-up study of their biological activities.

In order to explore the structure and biological iunction of SCD in pig, bioiniormatics software like Expasy and 3opma were used to analyze the amino acid sequence of SCD. The results showed that SCD protein of pig was composed by 359 amino acids, and had a predicted molecular weight of 41.38 ku. SCD had four transmembrane domains,respectively containing one potential O-glycosylation sites, three N-glycosylation sites and 16 phosphorylation sites. Alpha helix, beta turn, extended strand and random curl were the main components of secondary structure. The low complexity and transmembrance region were two functional structures of SCD.

A high-performance liquid chromatography （HPLC）-photodiode array detector （DAD） method was established for determination of paracetamol, dipyrone, phenazone, naproxen, aminopyrine illegally added into veterinary antibiotics injection and soluble powder. Samples were extracted under uhrasonication with methanol. A CAPCELL PAK MG Cis analytical column （4.6 mm ×250 mm, 5 μm） was used for the column temperature was at 30 ℃ and the detection wavelength was set at 229 nm. Acetonitrile-phosphate buffer was used as the mobile phase under the program of gradient elution at a flow rate of 1.0 mL/min. The calibration curves of paracetamol, dipyrone, phenazone, naproxen and aminopyrine showed good linearity in the ranges of 5.04-100.88, 12.04-240.72, 5.04-100.72, 2.02-40.40 and 5.02-100.32 μg/mL. The average recoveries （n=5） were 97.9%- 102.8%, 91.9%-98.8%, 96.2%-101.1%, 94.1%-102.3% and 96.0%-102.8%, and RSDs were 1.0%-3.l%, 0.9%-6.4%, 1.7%-4.2%, 1.4%-3.9% and 0.9%-4.8%, respectively. The methodology validation showed that this method was simple, accurate and reliable, so it was appropriate for the determination of the antipyretic analgesic drugs in many kinds of veterinary antibiotics.

In order to alleviate the effects of heat stress on the production performance of dairy cows and reduce the economic loss, the functional nutrition package against heat stress in dairy cows was prepared. One hundred and twenty healthy Holstein lactating cows were randomly divided into experimental group and control group according to the principles of similar parity, milk yield, lactating days and body condition. Under the conditions of moderate heat stress, the dairy cows in control group were fed with the basal diet, and the dairy cows in experiment group were fed with the basal diet with adding 300 g/（d· head）composite functional nutrition package. The anti-heat stress effects of composite functional nutrition package were observed. The results showed that the unit yield of dairy cows in test group was significantly higher than that in control group （P〈0.05） at the end of the test. Compared with pre-trail period, the average unit yield of dairy cows in control group reduced 2.96 kg/（d, head）and that in test group reduced 1.16 kg/（d· head）. The reduction of unit yield in test group was 1.80 kg/（d· head）lower than that in control group. The increasing amplitude of milk protein in test group was 0.13 percentage points higher than that in control group. The milk fat percentage and dry matter content in test group at the end of the test and on the 10-th day after the test were significantly higher than that in control group （P〈0.05）. The reduction of the feed conversion rate of dairy cows in test group was 0.02 lower than that in control group. The results suggested that functional nutrition package against heat stress could not only alleviate the reduction of milk yield caused by heat stress, but it could also enhance the milk protein content, milk fat percentage, dry matter content and feed conversion rate.

In order the understand the fineness changes of cashmere fiber of Alashan White Cashmere Goat in Alashan of Inner Mongolia in recent years, the fineness of cashmere fiber of Alashan White Cashmere Goat in Honggeereleng Region was determined in 2010 and 2014 using Automatic Finer Fineness Analysis Instrument by national standard method. The differences of cashmere fiber fineness of cashmere goat in different years were compared, and the distribution of cashmere fiber fineness was analyzed. The results showed that the average fineness of cashmere fiber of male and female Alashan White Cashmere Goat in 2010 were （16.60±1.63） and （16.93±1.58） μm respectively. The average fineness of cashmere fiber of male and female Alashan White Cashmere Goat in 2014 were（13.83±0.79） and （14.52±0.74）μm respectively, which was extremely significantly lower than that in 2010 （P〈O.O1）. The results showed that the fineness of cashmere fiber of Alashan White Cashmere Goat was significantly reduced by relevant measures taken in recent years.

Twenty Kunming mice were divided into five groups randomly （half male and half female） ,with four mice in each group, and the five groups were blank control group, barbed sugar group, argy wormwood leaf group, celery group and yoghurt group, respectively, and fed according to 0.1 mL/10 g dose, once a day, totally 15 days. The tail blood was collected for routine blood test before delivery, after 7 and 15 days. The results showed that the barbed sugar, argy wormwood leaf, celery and yoghurt could improve HB levels in mice, and celery could reduce lymphocyte ratio and increase the total number of olatelets.

In order to optimize the culture method in vitro of granule cells in Tibet yak, granule cells separated from follicular fluid and mature oocytes of Tibet yak were collected to make the culture test in vitro. The results showed that the formation of mono- layers of granule cells collected from follicular fluid and mature oocytes needed 6-7 d and 5-6 d respectively during the primary culture. During the subculture, two sources of granule cells started the adherence on the second day after the inoculation, dominant growth appeared from the third day to the fifth day, and granule cells paved on the dish bottom. The results showed that the culture in vitro with the granule cells separated from mature oocytes were simple and convenient, compared with granule cells separated from follicular fluid.

In order to study effects of different testing factors on the error rate of Bacillus licheniformis, the sample was treated by using different dilution methods and different media under different temperatures. The result showed that different dilution methods had minimal impact on the error rate of Bacillus licheniformis, the media in the second position. But it had great effects on the error rate of Bacillus licheniformis after treatment of 80 ℃ temperature for 10 minutes.

Effects of different nutrition levels （A 1 for 1.0 times NRC, A2 for 0.9 times NRC and A3 for 0.8 times NRC）and gender （B1 for male lamb and B2 for female lamb） on the production performance of Cele black sheep lamb and economic benefit were studied. The results showed that at the end of the test weight of the A1B1 group lamb was increased by 6.17%, 7.44%, 12.84%, 14.29% and 17.22% ,respectively, compared with that of A1B2, A2B1, A2B2, A3B1 and A3B2 group lambs,and average daily gain of the A1B1 group lamb was increased by 20.87%, 17.06%, 34.89%, 37.35% and 39.62% ,respectively ,compared with that of A1B2, A2B1, A2B2, A3B1 and A3B2 group lambs ;feed conversion ratio of the A1B1 group lamb was decreased by 28.24%, 23.96%, 62.47%, 63.84% and 62.96% ,respectively,compared with that of A1B2, A2B1, A2B2, A3B1 and A3B2 group lambs. According to economic benefit analysis, net income of the A1Blgroup lamb was increased by 17.13%, 28.19%, 41.58%, 40.24% and 42.69% ,respectively,compared with that of A1B2, A2B1, A2B2, A3B1 and A3B2 group lambs. The results indicated that Cele black sheep male lamb fed by 1.0 times NRC can obtain good production performance and higher economic benefit.

The single factor Randomization design was used, and four levels of VE were set, with three replicates each level. Total 96 1-day old AA broilers were selected, and maize-soybean meal basal diet was adopted. The levels of VE were 5, 15, 150 and 1 500 IU/kg. The nutrient levels of all diets were the same except VE. The period of test was four weeks. After the test finished, daily feed intake, daily gain, feed conversion ratio, phagocytic index, immune organ index and the antibody to Newcastle disease virus of AA broilers of each group were calculated. The results showed that adding 150-1 500 IU/kg VE could increase HI titers, spleen index and bursal index, but decreased the production performance. It indicated that VE requirement of 1-28 days old AA broiler chickens was 5 IU/kg.

[ Objective ] The research aimed to study the nutrition and stress situations of perinatal sows. [ Method ] 15 new-born crossbred sows of Landrace ×Large White with the same batch were selected to collect blood samples on the 14-th day before parturition, the parturition day and the 14-th day after parturition. The levels of serum cortisol, haptoglobin, nonesterified fatty acid （NEFA）, glucose, insulin and urea were detected. [Result] The cortisol concentration in the serum of the parturition day was significantly higher than that of the 14-th day before parturition and the 14-th day after parturition （P〈0.01）. The concentration of haptoglobin in the serum of the parturition day increased by 25.81% than that of the 14-th day before parturition （P〈0.05）. The level of NEFA in the serum of the parturition day was 3.54 times of that of the 14-th day before parturition（P〈0.01 ）and 3.63 times of that of the 14-th day after parturition （P〈0.01）.The insulin level in the serum of the parturition day was significantly lower than that of the 14-th day before parturition and 14-thh day after parturition （P〈0.01）. The urea level of the parturition day was 1.46 times of that of the 14-th day before parturition （P〈0.01 ）and significantly lower than that of the 14-th day after parturition （P〈O.01 ）. [ Conclusion ] Perinatal sows were under the physiological conditions of high-intensity stress, deterioration of health status and negative nutrition balance.

In order to know the effects of supplementing with protein feed and energy feed on the milk yield of grazing Alashan Bactrian camel, corn and concentrate supplement were selected to make the supplement experiment and observe its effects of increasing milk yield. The results showed that the increasing effects of milk yield of supplementing grazing Alashan Bactrian camel with protein feed was better than that with energy feed. The milk yield of Alashan Bactrian camel in test group 2 at the end of the test increased 277 g/（d. head ） than that before the test （P〈0.01 ）. The milk yields of Alashan Bactrian camel in energy feed group and control group were lower than that before the test.

Through the investigation on the grassland ＇no-rodent damage model district＇ from 2008 to 2010, the rodent population density, birds of prey and food kernel number, herbage yield and biomass were analyzed to explore the effect of biological control of pest. The results showed that： the number of effective rodent caves in all plots decreased from 2008 to 2010, and birds of prey and food kernel number per kilometer increased year by year; the grassland biomass in three years was increased by 27.7%, the vegetation coverage was increased by 43.8%, and the pasture height was increased by 22.9%; the establishment of scaffolding to control mice saved 43 697.4 tons of fresh forage loss total, and recalled the economic loss of 7 739 480 yuan; grassland biomass increasing value during the year was 8 739 480 yuan; the establishment of scaffolding used natural food chain and food structure factor net in grassland ecosystem to control rodents from the angle of ecology, could protect the ecological environment of grassland, improve grassland deratization effect so as to get the target of effectively controlling the rodent quantity for long tenn.

Based on four meteorological station＇s daily data in Jinzhou from 1971 to 2010, climate characteristics, type and path of cold wave were analyzed, and in particular, the cold wave from 2006 to 2010 was analyzed. The results showed as followed： cold wave happened in Western part more than Southern and Northeastern, and the cold wave mostly happened in October and November;cold wave happened in Northern earlier than Southern and Northeastern,but ended later; cold wave happened 2.6 times every year, and the number of cold wave happened downward trend year by year, which was consistent with global warming trend ;the cold wave affecting the city was divided into Northeast, Northwest and West path, and judging from the impact strength, Northeast and Northwest path were more intense than the West path, and cooling rate was slightly larger than the West path ; cold wave happened in Jinzhou region was divided into transverse groove, cold vortex type, a trough a ridge and a small groove development, among them the transverse groove maximum;through comparative analysis, it was found that 1-2 days before the cold wave outbreak, the temoerature of Jinzhou region generally rose 6-8 ℃.

With the development of molecular biology, the theoretical and application studies on single-chain antibody as a micro-molecule antibody prepared by genetic engineering have attracted wide attentions. And it showed considerable application prospects in imaging localization and diagnosis, targeted therapy and cellular immunity. The structure, characteristics and preparation technology of single-chain antibody as well as its applications were reviewed, so as to provide theoretical foundation for further study on single-chain antibody and its applications.

Amniotic fluid-derived stem cells （AFSCs） exist in the amnionic fluid of human and animals. AFSCs are generally acknowledged as a special cell type between embryo stem cells （ESCs） and adult stem cells （ASCs）, thereby, they have the biological characteristics of embryo stem cells and adult stem cells. The keeping of AFSCs characteristics is based on the whole specific expression of its marker gene and genome. At present some marker genes of AFSCs at different gestational ages like Oct4, Sox2, Nanog and SSEA have been studied, and expression characteristics of Tra-1-60 and Tra-1-81 in genome have been analyzed. Research progress on AFSCs was summarized to provide reference for research on amnionic fluid stem cells.

The neuroendocrine and immune systems formed intra-system and inter-system communication circuitry by similar ligands and receptors, which maintained the homeostasis of body. As potential immunomodulators, neuropeptides participated in various kinds of immunity-regulating functions. The distribution of neuropeptide somatostatin （SS） and its receptors in the immune systems and their immunoregulatory functions on the immunity were reviewed, so as to provide theoretical foundation for exploring the immunoregulatory mechanism of SS.

In order to stimulate students＇ interest in learning Freshwater Aquaculture course and improve the teaching effect, the teaching content, teaching methods and assessment methods of this course were explored and practiced. New advances and new technologies were introduced in the teaching content to stimulate the students＇ initiative. Multimedia and internet technology was comprehensively applied to enhance the teaching intuitiveness. The opening experiment content was set up and the innovation ability of students was enhanced. The assessment method was changed to inspire the learning motivation of students. Through the teaching reform of this course, the learning initiative and interest of students were enhanced, and the teaching quality was also improved.

The positive psychology emphasizes the people＇ s enthusiasm. The basic concept of positive psychology should be grasped in universities and colleges. The positive psychology should be introduced in the entrance education of college freshmen, so as to enhance the effectiveness of entrance education in universities and colleges. The basic concept of positive psychology and its significance for the entrance education of college freshmen were introduced. And some measures for promoting the entrance education of college freshmen by using positive psychology were proposed.

Traditional Chinese Veterinary Medicine is a required course of the animal medicine specialty in ordinary higher agricultural colleges. Due to its strong theoretical content and nonobjective language, it reflects Chinese ancient philosophy and causes the student to learn difficultly. Therefore, this article found some questions and provided the teaching methods through questionnaire survey to students of animal medicine specialty in order to improve the ability that the students treat disease with traditional Chinese veterinary medicine.

Experiment teaching is an important tache of achieving the target of talent cultivation in universities and colleges. As the plan model of cultivating excellent talents was proposed, the traditional experiment teaching mode of Veterinary Pathophysiology as a bridge course couldn＇t meet the cultivation target requirements of talents. Therefore, it was of important practical significance to further deepen and improve the experimental teaching reform of this course. The existing problems in the traditional experiment teaching were analyzed. And a reform scheme of experiment teaching in the plan model of cultivating excellent talents was proposed, so as to provide references for improving the experiment teaching effect of veterinary pathophysiology.

The main existing problems in the present practice teaching of Cattle Production in Tibet were analyzed and discussed. And some countermeasures were proposed. The practical effect of the practice teaching was summarized in order to attain the practice teaching objective of Cattle Production, so as to lay the foundation for cultivating the applied talents of animal husbandry in Tibet.

Summer high temperature heat stress can produce adverse effect on high yield production performance of laying hens. Effects of high temperature on feed intake, metabolism, digestion and absorption of nutrients, egg production, egg quality and immune function of laying hens were summarized, and countermeasures of adverse effects on laying hens were forwarded to provide a reference for the development of laying hens breeding.

The brands and varieties of prepacked children＇s food in 50 supermarkets and stores in Dongsheng District and three surrounding banners in Ordos City of Inner Mongolia were investigated. The results showed that there were 169 brands, 222 kinds of children＇s food in Ordos. Among them, 49 brands （29.0%）, 61 kinds（27.5%）were nutritious or nutrient supplement types. 120 brands（71.0%）, 161 kinds（72.5%） were leisure food or even junk food. There were 124 brands（62.3%）, 180 kinds（59.4%） of children＇s food sold in Dongsheng District of Ordos City. There were 75 brands（37.7%）, 123 kinds（59.4%）of children＇s food sold in three surrounding banners. These indicated that the economic and culture development levels in different regions were different, and the kinds and levels of children＇s food in different regions were different. The information about package and label was overall and the food in the survey had QS label. The labels of children＇s food in the survey were more qualified than that in the past years, but their quality needed to be detected.