Abstract: The aim of this study was to observe the biological characteristics of goat bone marrow mesenchymal stem cells （BMSCs）. Samples were collected from goat and the BMSCs were isolated and cultured. RT-PCR was used to verify the stem cell properties of the isolated cells by detecting the stem cell transcription factor oct4 gene and sox2 gene. Furthermore, the growth curve of the identified BMSCs was drawn. The results showed that the BMSCs exhibited primary fibroblast and were able to express the oct4 gene and sox2 gene, which proved its characteristics of stem cells. The growth curve of BMSCs was shown as ‘S’ type, the 1st and 2nd day when the cells were cultured were the incubation period, the 3rd day was the exponential growth phase, and the growth of the cells entered platform phase after the 7th day. It was indicated that the isolated cells were BMSCs and can be used for the subsequent study.