Abstract: In order to ensure the biosafety of mutton products, Toxoplasma gondii infection of slaughtered sheep was investigated and Toxoplasma gondii in mutton was detected. 677 sera samples of slaughtered sheep were detected by using indirect hem agglutination test for toxoplasmosis. The results showed that 47 sheep antibodies were positive and the infection rate of Toxoplasma gondii was 6.9%.