Abstract: In order to investigate the immunomodulatory effect of wolfberry polysaccharide on chicks, a total of 400 1 day old chicks were randomly divided into four groups （A, B, C, D）. When the chicks were 7 days old, the group A and B were gavaged with 5 mL of 10 mg/kg and 20 mg/kg of wolfberry polysacchafide respectively, the group C and D were gavaged with the same volume of saline. Meanwhile, the group A, B and C were vaccinated with Newcastle Disease virus IV strain vaccine by intraocular-nasal route, the group D were not vaccinated. The micro hemagglutination inhibition test was used to determine the antibody titer of blood serum of the four tested groups in 7, 14, 21, 28 and 35 days old, respectively, and the immune organ index of the four tested groups were calculated at the 7th, 14th, 21th, 28th and 35th day after vaccination, respectively. The results showed that the antibody titer of blood serum of the group A and B were higher than that of group C and D, indicating that the wolfberry polysaccharide could enhance the immune effect of chicks against Newcastle Disease virus.