Abstract: In order to determine the chitosan deposit level in selected tissues of carp after they fed with exogenous chitosan, a total of 210 carp were randomly divided into 7 groups, including control group and experimental groups Ⅰ-Ⅵ. The basic diet of experimental groupsⅠ-Ⅵwere added with 0.50%、1.00%、1.50%、2.00%、3.00%and 4.00%chitosan, respectively, the control group fed with routine diet without exogenous chitosan adding. During the 60 d of formal test period, one carp was randomly selected in each replicate from the control group and experimental groups every 10 d respectively to determine the chitosan deposit level in scale, fin and myoideum. The results showed that with the extension of feeding time and the increase of adding amount of exogenous chitosan in feed, the chitosan deposit level in all tested tissues for control group and experimental groups were increased in different degrees. However, no significant differences were observed in the change of chitosan deposit level in scale when the adding amount of exogenous chitosan in feed exceeded 3.00%, and there were no significant differences of the increase of chitosan deposit level in fin when the adding amount of exogenous chitosan in feed exceeded 1.50%.In the same experimental period, there were no significant differences of chitosan deposit level in myoideum between control groups and experimental groups or among the experimental groups. It was indicated that when the adding amount of exogenous chitosan in feed reached certain degree, the chitosan deposit level in scale and fin would not significantly increase with the increase of adding amount. Furthermore, the chitosan deposit level in myoideum was less influenced by exogenous chitosan in feed with different adding amount.