In order to select and breed fine varieties of Trifolium strains, the seed color, seed size, thousand seed weight, germination rate and seedling emergence of Trifolium ambiguum Bieb., Trifolium repens L. and their ten F1 strains were compared and analyzed in this study. The results showed that significant differences were observed in all of the tested index of the among all tested materials （P〈0.05）. The seed characteristics of ten F1 strain were similar to those of female parent Trifolium ambiguum Bieb., and had great differences with those of male parent Trifolium repens L..

This study was conducted to investigate the effect of TBT at environment concentrations on the bodyweight and feeding related behaviors of root voles. The root voles of experimental group were administrated with 1μg/mL of TBT according to the dose of 5μL/（g＆#183;BW） once every 3 days by gavage, and those of control group were administrated with the same volume physiological saline. During the experiment period of 45 d, the body weight of the root voles was recorded and the amount of their food intake and drinking water （supply-surplus） were measured. Furthermore, the duration and frequency of feeding, drinking and movement were recorded with videotape. The results showed that only in a limited number of observation time points, the duration and frequency of feeding and drinking of male root voles in TBT exposure group were significantly higher than those in control group （P〈0.05）. However, there were not significant differences of the duration and frequency of feeding and drinking between female root voles in TBT exposure group and those in control group. The movement time of male root voles in the 3rd d after administrated with TBT was significantly more than that of the male root voles in control group （P〈0.05）, whereas the rest time was significantly less （P〈0.05）. With extension of the exposure time, none of significant differences of the movement and rest time were observed between the two groups. The influence of TBT exposure on the amount of food intake and drinking water and body weight of female root voles was not significant. It was indicated that the body weight of adult root voles was not significantly influenced by TBT exposure at environment concentrations, whereas the feeding related behaviors were partly influenced.

In order to determine the chitosan deposit level in selected tissues of carp after they fed with exogenous chitosan, a total of 210 carp were randomly divided into 7 groups, including control group and experimental groups Ⅰ-Ⅵ. The basic diet of experimental groupsⅠ-Ⅵwere added with 0.50%、1.00%、1.50%、2.00%、3.00%and 4.00%chitosan, respectively, the control group fed with routine diet without exogenous chitosan adding. During the 60 d of formal test period, one carp was randomly selected in each replicate from the control group and experimental groups every 10 d respectively to determine the chitosan deposit level in scale, fin and myoideum. The results showed that with the extension of feeding time and the increase of adding amount of exogenous chitosan in feed, the chitosan deposit level in all tested tissues for control group and experimental groups were increased in different degrees. However, no significant differences were observed in the change of chitosan deposit level in scale when the adding amount of exogenous chitosan in feed exceeded 3.00%, and there were no significant differences of the increase of chitosan deposit level in fin when the adding amount of exogenous chitosan in feed exceeded 1.50%.In the same experimental period, there were no significant differences of chitosan deposit level in myoideum between control groups and experimental groups or among the experimental groups. It was indicated that when the adding amount of exogenous chitosan in feed reached certain degree, the chitosan deposit level in scale and fin would not significantly increase with the increase of adding amount. Furthermore, the chitosan deposit level in myoideum was less influenced by exogenous chitosan in feed with different adding amount.

In order to understand the characteristic of change of MICs of different antimicrobial agents against the resistant E. coli strains induced by certain antibiotics in vitro, induction experiment of E. coli ATCC 25922 was conducted using tetracycline hydrochloride, ciprofloxacin hydrochloride, sodium salicylate and chloramphenicol as inducers by 1/2×MIC method. Prior to the induction experiment, the tested strain was morphologically and biochemically identified. During the induction experiment, the tested strains were morphologically identified. The MICs of 16 antimicrobial agents against the quality control strain were determined ahead of induction, and the MICs of 16 antimicrobial agents against the 4 induced strains were also determined when the concentrations of inducers reached to 128×MIC. The results showed that the morphological and biochemical characteristics of the tested strains were identical to E. coli. Before induction, the MICs of 16 antimicrobial agents against the quality control strain ranged from 0.25 to 64.00 μg/mL. MICs of cefotaxime, tetracycline hydrochloride, oxytetracycline, thiamphenicol, norfloxacin, cefoxitin and some of other antibiotics against the tetracycline hydrochloride induced strains were increased by 4 fold or more than 4 fold compared with those against the quality control strain, which showed a statistical significance. While MICs of cefotaxime against the ciprofloxacin hydrochloride induced strain and chloramphenicol induced strain were increased by more than 4 fold compared with that against the quality control strain, no statistical significances were found in the change of MICs of other antibiotics against the ciprofloxacin hydrochloride induced strain and chloramphenicol induced strain. When the concentration of sodium salicylate exceeded 16×MIC, the induced strain were not able to grow. It was indicated that the change of MICs of the tested antibiotics against the tetracycline hydrochloride induced strain was most obvious, and the E. coli was prone to become resistant under the selective pressure of tetracycline.

Artificial controlled lighting technology plays an important role in the breeding process of fur animals. Here we introduced the methods of artificial controlled lighting commonly used in fur animals breeding and reviewed the effect of artificial controlled lighting technology on growth and development of fur animals, so as to provide theoretical support for the scientific breeding of fur animals.

To investigate the effects of infectious bovine rhinotracheitis virus （IBRV） infection on phagocytizing chicken erythrocytes function of bovine monocyte-macrophage, lymphocyte separating medium was used to isolate the bovine peripheral blood mononuclear cells. The isolated cells were subsequently cultured to monocyte-macrophage by adherent culture method. The chicken erythrocytes were added to bovine monocyte-macrophage which was infected by IBRV for 24 h, and the phagocytizing chicken erythrocytes rate of the infected cells was determined. The results showed that the phagocytic chicken erythrocytes rate of IBRV infected bovine monocyte-macrophage was significantly reduced （P〈0.05）.

The species number and abundance of the avifauna communities in north campus of Xichang College were surveyed by line-intercept method during November 2013 to January 2014. The results showed that a total of 57 species were recorded which belong to 26 families and 8 orders. The dominant species were Pycnonotus cafer, Egretta garzetta and Passer montanus. Among the 57 species, 17 species （29.82%） belonged to the palaearctic realm, 29 （50.88%） species belonged to the oriental realm and other 11 species （19.30%） were widespread species. The avifauna floristic composition showed obvious oriental characteristics. According the survey, there were 40 （70.17%） residents, 2 （3.51%） winter residents, 8 （14.04%） summer residents and 7 （12.28%） travelers in this campus. Two species were under special provincial protection, 46 species were enrolled in the list of beneficial species with economic and scientific research values of China, and 1 species were enrolled in the appendix Ⅱ of CITES. The most （49 species） birds were recorded in the habitat of shrub and forest. Secondly, 33 species were recorded in open forest and shrub communities. The birds of Passeriformes accounted for the highest ratio in the turf habitat（84.66%）.

Based on measure formula of species diversity, the plant species and composition characteristics of herbage and shrub communities in floodplains wetland of suburb of Xichengyi of Ganzhou District of Zhangye City in Heihe River basin were investigated. The results showed that Phragmites communis was the dominant species of the herbage community, and the important value was 24.16. Typha minima was the dominant species of shrub community, and the important value was 47.54. The results of species diversity analysis of the two communities showed the consistent trends that the diversity index values were relatively lower. Furthermore, the abundance degree, Simpson diversity index, Shannon-Wiener index and uniformity index of shrub community were all higher than those of herbage community, whereas the dominant index of shrub community were higher than that of herbage community. The results of variance analysis showed that there were no significant differences of species diversity index between the two communities, indicating that the structure of the two communities had not obvious changes. It was indicated that the plant structure of the investigated communities were simple and the plant diversity were low, whereas the advantage phenomenon were serious.

Bursa of Fabriciu is the central immune organ of avian and is also the place for development, differentiation and maturation of B lymphocytes. In sexual maturity, the structure of Avian bursa of Fabriciu was degenerated and was covered by connective tissue with rich vascular and fat. In order to further understand the mechanism of age-related involution of avian bursa of Fabricius, the research progress on histological characteristics, age-related involution and neuroendocrine regulation of avian bursa of Fabricius were reviewed in this paper.

The R-spondin （Rspo） protein family is a newly found secretory protein family in recent years which include four family members （Rspol to Rspo 4）. All the members in Rspo protein family contain four highly conserved domains： hydrophilic secretion signal peptide domain in N-terminal, two or three furin-like cysteine-rich domains, thrombospondin-type 1 （TSP1） domain and amino acid-rich basic domain in C-terminal. Wnt signaling pathway is one of the significant pathways in organism which plays a crucial role in the process of growth and development. As a member of Rspo protein family, Rspol is a potential female-determination factor that can activate Wnt signaling pathway and regulate the sex-determination and other biological function of organism by cooperation with Wnt ligands.

The Tibet grassland culture, originated from Tibet plateau, has a long history and is an important part of Chinese grassland culture. After the influence and promotion by original indigenous culture, Qiang culture, ZhangZhung culture, Tuyuhun culture, Tibetan culture, Tang culture, Buddhist culture, Mongolia culture and many other cultures, the unique Tibet grassland culture had been formed. Its specific features include adaption to the regional characteristics of plateau, multiple ethnic fusion of the national characteristics and bright religious characteristics. Here we introduced the formation and characteristics of Tibet grassland culture.

In order to select forage varieties suitable cultured in San Jiang Yuan area and to develop culture techniques, the planting test of Vicia sativa L. and oats was carried out in Xinghai County, Tongde County, Yushu County and Maqin County of Qinghai Province. The results showed that the most yield of forage （4 799.76 kg/667 m^2） were produced by mixed sowing with Vicia sativa L. and oats in Tongde County. It was indicated that forage culturing in San Jiang Yuan area could produce higher yield and the economic benefits were significant.

Silage fermentation is a complex process, including a series microbial activity and biochemical changes. The additives are frequently used to regulate the silage fermentation. In order to provide references for the future research of silage additives, the utilization and research status at home and abroad of additives employed in silage were reviewed in this paper, with emphasis on lactic acid bacteria, enzymes and molasses additives.

In this paper, the preparation methods of corn stalk silage in high altitude areas, including constraction of silage cellar, preparation of corn stalk, placement of corn stalk into silage cellar, planishing corn stalk silage mound, sealing of silage cellar, terminal time of silage fermentation, test of corn stalk quality （character, odor, color, pH value, etc.） were introduced,and the silage feeding technology were also reviewed, so as to provide references for the full utilization of local corn and other forage resources and domestic animal breeding.

The feeding effect of microbial fermented corn stalk on Corriedale sheep was evaluated in this study. The result showed that the daily gain of sheep feed with microbial fermented corn stalk was 23%increased than that of sheep feed with dry corn stalk （P〈0.05）,7% increased than that of sheep feed with hay. Furthermore, the daily net profit produced by per sheep feed with microbial fermented corn stalk was 0.78 and 0.42 yuan more than that of the sheep feed with dry corn stalk and the sheep feed with green dry hay, respectively. It was indicated that microbial fermentation of corn stalk is an important technique worthy of promotion in Bijie City of Guizhou province.

In order to cultivate qualified talents of animal husbandry and veterinary medicine major, the bilingual teaching model of the culture of animal husbandry and veterinary medicine major talents were discussed in this paper, so as to further promote the reform of bilingual teaching and improve the teaching quality of bilingual teaching. Based on the characteristics of animal husbandry and veterinary medicine major, a series of reforms in teaching model, selection of text book and teaching effect evaluation model of bilingual teaching of Animal Physiology were performed. The teaching practice showed that most of the students are satisfied with the effect of bilingual teaching.

Facilities Horticulture is one of the major professional courses in professional degree graduate of horticulture field. In this paper, the revisions basis and content of syllabus of Facilities Horticulture are determined by analysis of significance of syllabus in classroom teaching and its actual application effect, and the teaching actual effect after the revisions of syllabus of Facilities Horticulture is elaborated.

As significant support and supplement for Anirnal Physiology theoretical teaching, Animal Physiology Experiment is an important measure to evaluate the effect of theory teaching and consolidate the effect of classroom teaching. Taken applied talents culture as aim and practice ability and innovation ability culture of the students as core, exploration of teaching reforms were performed on the aspects of teaching contents, teaching methods and evaluation mechanism to improve the teaching quality of experimental teaching.

Animal Anatomy Experiment is an experimental course and its content is mainly involved in animal operating. The biological safety problems existing in animal anatomy experiment are indispensable. In order to avoid the occurrence of biological safety problems in Animal Anatomy Experiments teaching, some suggestions were proposed in this paper based on the experiences in Animal Anatomy Experiments teaching.

Animal Quantitative Genetics is a professional basic course of animal science major and plays an important role in teaching of animal science major related courses. Recently, with the development of computer technology, multimedia technology has been widely applied in the teaching of Animal Quantitative Genetics. In this paper, the advantages and disadvantages of application of multimedia technology in Animal Quantitative Genetics teaching were analyzed and the advises for improvement of teaching quality in future were proposed combining with the teaching practice, so as to make better use of multimedia technology for the teaching ofA nimal Quantitative Genetics.

The Germany ＂dual system＂ education mode focus on the culture of students＇ vocational skills, and the teaching and studying form are flexible and various. Furthermore, the curriculum emphasizes on the production requirements of the market, and the graduation and employment are seamless docking. Lots of researches and practices on Germany ＂dual system＂ education mode has been conducted since it was introduced to China and certain achievements have been obtained. However, duo to the particularity of national conditions, there are few successful application eases of ＂dual system＂ education mode in Chinese secondary professional school. In order to provide references for the flexible promotion of ＂dual system＂ education mode in China, the regional differences, resource differences and economic differences were analyzed and discussed in this paper.

Agricultural microbiology is a required and specialized basic course of cultivation and identification of Chinese herbal medicine major which occupies an important position in the curriculum system. Combined with the characteristics of Agricultural Microbiology and the teaching reform experiences of this course for years, the reform of teaching methods and examination form was performed to clearly understand the of students＇ mastery of knowledge and cultivate the students＇ practical ability.

Sheep pen is an important place for sheep to exist, live and produce. The design and establishment of sheep pen have great influence on large scale and intensive farming in different regions. Based on the climate characteristics in most areas in southern China, such as rainy and humid, warm in winter and spring, and torrid in summer and autumn, the establishment of standard sheep pen was introduced on the aspect of site selection, plan the scale of construction, principles, layout of structure and construction of sewage disposal facilities combining with physiological requirements and behavior habits of sheep to provide some references.

The methods recently used at home and abroad for quantitative detection of melamine in dairy products and feed, including chromatography method, chromatography-mass spectrometry method （GC-MS,LC-MS）, spectrometry and immunoassay method were introduced in this paper. By analysis and comparison of these typical methods, it was found that on one hand, some of these detection methods were expensive and their employment was limited to laboratory detection and was difficult to promotion in routine test; on the other hand, while the cost of some of detection methods was relatively low, their detection results were not reliable. However, introduce and employment of nano-gold, grapheme and other novel materials made trace detection of melamine more convenient, accurate and faster.

Intestinal Diseases are the most frequently prevalent diseases in broilers under the high humidity climate from March to May in southern China. The symptoms of these diseases are different types of diarrheas. In order to provide effective prevention and control measures of broilers intestinal diseases, the etiology of these diseases were analyzed and the measures, such as feeding diet with low level of protein and metabolizable energy, strengthening management, employing Chinese herbal medicine to regulate intestinal function and feeding river sand to promote digestive function were proposed in this paper.

Nineteen cases of enteritis type canine parvovirus disease in an animal hospital were diagnosed based on the analysis of the treatment protocol and experience. Among the cases, two of them were mixed infection with parasite, four of them were mixed infection with coronavirus, and other 13 cases were simple infection of parvovirus. The sufficient and prompt employment of antiviral drugs combining with anti-inflammation, fluid infusion and correcting the unbalance between acid and alkali were effective treatment measures for canine parvovirus disease.