Abstract: In present study, the antimicrobial resistance of 26 strains of Streptococcus suis isolated from veterinary clinical specimens was investigated by K-B method recommended by CLSI. The results showed that most （73.0%） of the isolates were sensitive to eefotaxime and ampicillin/sulbactam. Furthermore, 57.7% and 50.0% of the isolates were sensitive to amikacin and doxycycline hydroehloride, respectively. The high rate of resistance to tetracycline （92.3%）, gentamiein （69.2%） and sulfadimethoxine （69.2%） was observed, and 57.7% of the isolates exhibited resistance to chloramphenicol, cefiazidime, clindamyein, sulfadiazine sodium, and trimethoprim-sulfamethoxazole. The Streptococcus suis isolates have been found resistant to the antimicrobial agents commonly used in veterinary clinic, which should be paid more attention.