Abstract: Abstract ：The skin in different body parts of Rana catesbeiana were collected to prepared paraffin sections by using HE staining method and were histologically observed. The thickness of skin in different body parts was determined by using image processing software of hnage J 4.21 and was statistically analyzed by One-Way ANOVA analysis of variance using SPSS 17.0 statistical software. The skin of Rana cazesbeiana consisted of epidermis and dermis. The epidermis could be divided into 3 layers from outside to inside, including cuticle, granular layer and germinal layer. The dermis was composed of loose layer and dense layer. The loose layer was primarily composed of reticular connective tissue and also included some pigments, granular glands and mucous glands. The pigments were mainly distributed in subcuticular connective tissue, and a small amount of pigments were distributed in the connective tissue in gaps between granular glands and mucous glands. The value of thickness of tympanic skin was significantly lower than that of skin in the other parts of Rana catesbeiana （P〈0.05）. No significant differences in thickness of skin in forelimb, hind limb and cloacal pore were observed （P〉0.05）. The distributing density of pigment cells, granular glands and mucous glands varied in skin in different body parts of Rana catesbeiana. The number of mucous glands distributing inside was more than that outside, and the granular glands were mainly distributed in epidermis but barely in deep layer.