Abstract: Enrofloxacin, belonging to the third generation of fluoroquinolone, is a kind of antibiotic with broad antibacterial spectrum and is widely applied in livestock and poultry breeding and aquaculture. With the increasing amount of enrofloxacin used in agriculture production, its effect on cytochrome P450 enzyme system is also widely investigated. Here we introduced the basic characteristics of cytochrome P450 enzyme and reviewed the research progress on effect of enrofloxacin on cytochrome P450 enzyme system, so as to provide reference for further understanding of interaction mechanism between enrofloxacin and cytochrome P450 enzyme system as well as pharmacologic and toxicologic features of enrofloxacin in livestock and poultry.