Abstract: In order to effectively prevent the occurrence of diseases of Litopenaeus vannamei and to increase the comprehensive benefit of pond culture, the technique for polycuhure of Litopenaeus vannamei, Ctenopharyngodon idellus and Cyprinus carpio was developed. The output production of Litopenaeus vannamei, Ctenopharyngodon idellus and Cyprinus cazpio under the polyculture condition was 3 600, 3 300 and 2 400 kg, respectively. The production value was 116,700 Yuan per hectare, and the net profit was 40,800 Yuan per hectare. The mode of polycuhure of different aquatic animals was effective for the utilization of water space; The ability of self-cleaning of the water in pond was enhanced; The transmission route of aquatic animals diseases was effectively blocked, avoiding the outbreak of the diseases. The risk of the aquaculture was reduced and the economic benefit was increased.