Abstract: A method for determination of organic carbon and organic matter in animal manure was established in this study, which was subsequently used to assess the content of organic carbon and organic matter in animal manure samples collected from 16 intensive livestock and poultry farms in Beijing. We found that the content of organic carbon and organic matter varied in manure samples from different animal species. The highest（35.15%） and lowest（15.14%） content of organic carbon were found in porcine manure sample and bovine manure sample, respectively. Whilst, the highest（70.76%） and lowest（32.58%） content of organic matter were observed in chicken manure sample and bovine manure sample, respectively. The correction equation for determination of organic carbon and organic matter in animal manure established in this study was： w（OC） = 0.5129w（OM）-2.3377, and R~2 was 0.9255.