Abstract: A total of 150 non-immunized 4-7 months old sheep from 15 raising households of Suide County of Shannxi Province were included in this study to compare the immune effect of different immune methods for foot and mouth disease. All of the selected sheep were singly vaccinated with inactivated foot and mouth disease vaccine. Subsequently, 30 of the vaccinated sheep were subjected to booster immunization with the same vaccine. The antibody against foot and mouth disease virus in singly immunized and booster immunized sheep were determined. The results revealed that the qualified rate of antibody level in singly immunized sheep was 55.33% and that of booster immunized sheep was 93.33%. It was indicated that the immune effect of booster immunization method is superior to that of single immunization.