Abstract: JIVET is a technique using reproductive hormone to stimulate ovarian of young stock to rapidly and efficiently produce oocytes. In this study, in order to evaluate the application performance of JIVET in rapid propagation of Suffolk Sheep, 4 one-month-old lambs of Suffolk Sheep were selected and intramuscularly administrated with FSH and PMSG. Forty-eight hours after administration of reproductive hormone, the oocytes were collected by surgery and were subsequently matured in vitro. The matured oocytes were fertilized with prepared frozen semen in vitro. When the embryos grew to 2 to 8 cells each, they were transferred into the uterus of 6 receptor female sheep. The results revealed that a total of 109 available oocytes were obtain after the treatment of reproductive hormone and 79 of them were successfully matured in vitro, and the maturation rate was 72.48%. A total of 34 cleaved embryos were obtained after fertilization in vitro and the cleavage rate was 43.04%. Three receptor female sheep was pregnant after receiving embryo transfer and the pregnancy rate was 50%. Finally, 3 lambs of Suffolk Sheep were obtained. These results suggest the fact that the application of JIVET can shorten the breeding cycle of Suffolk Sheep and thus speed up the breeding of Suffolk Sheep.