Abstract: It is well known that endocrine disrupting chemicals（EDCs） is harmful for the health of animals and human. Most of the EDCs eventually enter into the water environment and cause damage of reproductive system and endocrine system of aquatic organisms. In the present study, the Gobiocypris rarus was used as material to evaluate the effect of MT exposure on its hormone levels. The concentrations of E2, T and 11-KT in adult female and male Gobiocypris rarus after exposure of MT for 7 days were determined by ELISA. The results revealed that compared to control groups, the concentrations of E2, T and 11-KT in female fish exposed in MT were significantly up-regulated（P〈0.01）; however, in male fish exposed in MT, the concentrations of E2, T and11-KT were significantly down-regulated（P〈0.01）.