Abstract: The aim of the present study was to clarify the migration processes and rules of environmental antibiotics in the soil-plant system. The sulfamethoxazole （SMX） was used as target antibiotic to characterize its content variation in soilless cultivated pakchoi, Malabar spinach and Chinese cabbage during a 24 h root exposure. The concentration distribution of SMX in different parts of the selected vegetables after 24 h root exposure was statistically assessed and the accumulation rate of SMX in the leave of the vegetables was calculated to evaluate the migration rules of SMX. The results revealed that root exudates showed no effect on absorption of SMX in root of pakchoi and Chinese cabbage, but they showed effect on SMX transmission from root to leave;root exudates not only promoted the absorption of SMX in root of Malabar spinach, but also showed effect on transmission of SMX absorbed from root to leave;29.6-383.2μg/kg of SMX could be determined in the leave of the selected vegetables after 1 h root exposure;the accumulation rate of SMX in the leave of the vegetables was generally reduced with the extension of root exposure time, and it varied in different varieties of vegetables. The combined data suggests that the concentration of SMX in root and root exudates may have influence on the migration of SMX to the plants.