Abstract: The aim of the present study was to predict the exon-intron structure of gene coding connectin in oyster and to determine its primary structure and domains. The exon-intron structure of gene coding connectin was deduced based on oyster genomic data by using bioinformatics method; the mRNA composition of coding regions for connectin in oyster was predicted; the primary structure of oyster connectin was determined and its domains were detected; phylogenetic tree was constructed by comparing amino acid sequences of homologous protein associated with connectin from different species. The results showed that the gene coding connectin in oyster genome covered 200 kb, the connectincoding mRNA was about 74 kb, and the relative molecular weight of oyster connectin was deduced as 2 700 ku; oyster connectin was composed of Ig, Fn, and kinase domain, PEVK domain and unique sequences; the oyster eonnectin phylogenetically belonged to protostome group. Oyster connectin is a new type of connectin-like protein.