To understand the allelopathic potential of Cenchrus pauciflorus, the allelopathy of different concentrations （0,25%, 50% ,75%, 100% ） of seed aqueous extracts from Cenchrus pauciflorus on seed germination of Bromus inermis, Phleum pratense and Agropyron cristatum was assessed by bioassay. The results showed that the seed aqueous extracts from Cenchrus pauciflorus had effects on seed germination and seedling growth of the three gramineous grasses in different degrees. The seed aqueous extracts from Cenchrus pauciflorus had no significant effects on germination potential and germination index of Bromus inerrnis, but had significant effects on its ratio of root to sprout. The seed aqueous extracts from Cenchrus pauciflorus had no significant effects on germination rate, germination index and germination potential of Phleum pratense, but had significant effects on its ratio of root to sprout （P〈0.05）. The seed aqueous extracts from Cenchrus pauciflorus had significant effects on germination rate, germination index, germination potential and ratio of root to sprout of Agropyron cristatum （P〈0.05）; the germination rate of Agropyron cristatum seed in 100% seed aqueous extracts from Cenchrus paucifloru was 18.6%, which was decreased by 56.8% than that of control; the germination potential and germination index was 6.7% and 3.24, which was decreased by 39.3% and 8.94 than that of control, respectively.

The aim of the present study was to predict the exon-intron structure of gene coding connectin in oyster and to determine its primary structure and domains. The exon-intron structure of gene coding connectin was deduced based on oyster genomic data by using bioinformatics method; the mRNA composition of coding regions for connectin in oyster was predicted; the primary structure of oyster connectin was determined and its domains were detected; phylogenetic tree was constructed by comparing amino acid sequences of homologous protein associated with connectin from different species. The results showed that the gene coding connectin in oyster genome covered 200 kb, the connectincoding mRNA was about 74 kb, and the relative molecular weight of oyster connectin was deduced as 2 700 ku; oyster connectin was composed of Ig, Fn, and kinase domain, PEVK domain and unique sequences; the oyster eonnectin phylogenetically belonged to protostome group. Oyster connectin is a new type of connectin-like protein.

The aim of the present study was to develop a multiplex RT-PCR for simultaneous detection of FMDV serotypes O, A and Asia-1. Four specific primers were designed by Primer Premier 5.0 Software targeting VPI gene of FMDV. The annealing temperature and other reaction conditions were optimized. The results showed that the detection limit of the established method was 1 pg/μL; the detection results of CSFV, PPV, PRV, PRRSV, PCV and other related virus by using the developed method was all negative, indicating the good specificity of the method. FMDV serotypes O, A and Asia-1 could be accurately discriminated by the multiplex RT-PCR method developed in this study which was suitable for rapid detection and molecular epidemiological survey of the three serotype of FMDV.

A QuEChERS Kit-UPLC/MS/MS method for simultaneously determination of 18 quinolone residues in livestock and poultry meat was developed in this study. The samples were oscillated and centrifuged in a 1% aqueous solution of acetic acid and acetonitrile （20：80, V/V）. The obtained supernatant was purified after transferring to purification pipe and was then concentrated to dry. The residues were dissolved in aqueous solution of methanol （methanol ：water=1：4） and were then filtered. The Cls chromatographic column （100 mm×2.1 mm, 1.7 μm） was used to isolate, and the 0.2% aqueous solution of formic acid and acetonitrile solution containing 0.2% formic acid were used as mobile phase to gradient elute. The 18 quinolone residues in livestock and poultry meat were qualitatively and quantitatively determined by UPLC/MS/MS. A good linear relationship of the 18 quinolone in the range of 0.5-80 ng/mL was observed, and the correlation coefficient （r） was all above 0.99. Recovery test was performed with three concentration levels of 5.0, 10.0, 50.0 μg/kg. The average recovery of the 18 quinolone ranged from 60.5% to 97.0%, the relative standard deviation ranged from 2.1% to 19.5%, and the detection limit of the method was 5.0 μg/kg. The developed method with good reproducibility, high sensitivity, short analysis time and strong ability of confirmation was suitable for the simultaneous determination of 18 quinolone residues in livestock and poultry meat.

[Objective]To establish a HPLC method for determination of lignan in flaxseed. [Methods]The samples were treated with liquid-liquid extraction; the standard substance of lignan was used to conduct quantitative analysis; Agilent Cls Sym chromatographic column （250 mm×4.6 mm） was used to isolate; aqueous solution of methanol （methanol：water=4：6） was used as mobile phase; lignan were detected by UV detector with wavelength of 290 nm; methodology validation were conducted by using standard addition recovery test and precision test. [Results]The recovery of lignan in flaxseed ranged from 95% to 108%, and the RSD of precision was 7.43%. The rechecked results of determination of lignan in flaxseed by the established HPLC method from different laboratories were similar, which proved the good repeatability of the method. [ Conclusion ]The established HPLC method could be used for determination of lignan in flaxseed and provided reliable experimental basis for the industrial lignan purification.

In intensive sheep breeding, there are disadvantages such as low efficiency, heavy workload and high cost in traditional sheep artificial restraining method. A novel type of sheep auto-adjust restraining device with simple structure was developed, which was highly automated, convenient for operation, labor saving and practical for usage. In order to evaluate its applicative effect in sheep production, the time consuming in Brucella vaccine injection and weighing by using developed auto-adjust restraining device was compared with that by using traditional artificial restraining method. The work efficiency of Brucella vaccine injection and weighing was increased by 3 times and 1.5 times, respectively, indicating that the production efficiency was improved; the developed auto-adjust restraining device prevented sheep from competing for food and separated workers from sheep. The development of sheep auto-adjust restraining device is of great significance for the improvement of comprehensive production and promotion of persistent and healthy development of animal husbandry industry. The device has the potential to be promoted and applied.

In order to assess the egg quality of Lhasa White Chicken, a total of 40 eggs from 43-week-old Lhasa White Chicken were randomly selected to determine their nutritional components. The results showed that the water content of Lhasa white chicken egg was 72.50 g/100 g, the protein content was 13.00 g/100 g, the fat content was 9.87 g/100 g, the phospholipid content was 5.47×10^4 mg/kg, and the cholesterol content was 2.59×10^2 mg/100 g; the total content of sixteen amino acids was 11.96 g/100 g, and the content of leucine （0.98 g/100 g） was the highest in essential amino acids; the total content of ten fatty acids was 96.20%, and the C14：0, C16：0, C16：1, C17：0, C18：0, C18：1, C18：2, C18：3, C19：0, C22：6 accounted for 0.35%, 25.74%, 2.21%, 0.20%, 9.87%, 37.32%, 19.18%, 0.44%, 0.19%, 0.70%, respectively; the content of nine mineral elements were Zn （12.70 mg/kg）, Cu （0.63 mg/kg）, Fe （22.30 mg/kg）, Mg （1.04×10^2 mg/kg）, Mn （0.35 mg/kg）, K （1.35×10^3 mg/kg）, Na （1.34×10^3 mg/kg）, Ca （6.30×10^2 mg/kg）, P （2.08×10^3 mg/kg）.

Three-way crossbreeding trials were conducted using Dorper ram, Poll Dorset ram, German mutton merino ram and ewe hybrid of Small-tail Han and Suffolk, respectively. The productive performance of the hybrids was determined. The results showed that the pre-slaughter empty body weight, carcass weight and slaughtering percentage of hybrids of Dorper×Small-tail Han and Suffolk reached 43.0 kg, 22.4 kg and 52.07%, respectively, and the three indicators were all higher than those of hybrids Poll Dorset×Small-tail Han and Suffolk and German mutton merino ×Small-tail Han and Suffolk; the daily gain of hybrids of Dorperx Small-tail Han and Suffolk reached 262.3 g which was higher and significantly higher than that of hybrids of Poll Dorset×Small- tail Hart and Suffolk and German mutton merino×Small-tail Han and Suffolk, respectively.

Retinol-binding proteins 4 （RBP4） is an important protein produced by progesterone receptor. It stimulates the expression of TGF and involves in the early development of embryo and the transportation of VA. RBP4 gene is a candidate gene for the reproductive performance of domestic animals and is widely studies in sheep, swine and bovine. The research progress on function of RBP4 in domestic animals at home and abroad in recent years is reviewed in this paper.

The aim of the present study was to understand the status of veterinary drug residues in milk and dairy products in Hohhot. Field investigation and questionnaire survey were conducted to investigate the sales of milk and dairy products from 65 supermarkets and 60 milk products specialty stores; the consumption of milk and dairy products of 139 residents in Hohhot was investigated; the status of veterinary drug use in clinic was investigated by the information collected from 35 veterinarians and 10 experts. A retrospective survey was conducted to investigate the detection results of veterinary drug residue of 370 milk samples from Hohhot by authoritative testing institution using LC-MS-MS, ELISA and other methods from 2014 to 2015. The veterinary drug residues of 74 dairy product samples in Hohhot were detected by HPLC. The results showed that 96.41% of the respondent in Hohhot consumed milk and dairy products, 56.11% of consumers concerned the problem of veterinary drug residue in dairy products; 94.46% of the milk sold in market of Hohhot was provided by the local dairy farm, and the other 5.54% was provided from other areas; 94.44% of the investigated dairy farms had full-time veterinarians; the veterinarians in 20% of the surveyed dairy farms had overused veterinary drug; 85.71% of the surveyed dairy farms had medication records; 94.29 % of the surveyed dairy farms strictly enforced the veterinary drug withdrawal period; all of the surveyed dairy farms tested the antibiotics in raw milk; currently the most commonly used antimicrobial drugs are penicillin, ampicillin, amoxicillin and cephalosporins followed by gentamicin and metronidazole. Laboratory tests results showed that the residues in milk produced in Hohhot from 2014 to 2015 were all negative and no residue was detected in the samples of commercial dairy products. The production and distribution of milk and dairy products in municipal districts of Hohhot are in line with the requirements of the maximum residue limits of veterinary drugs in animal foods.

The Calligonum L. is one of the most ecologically important resources in desert areas of China and is commonly used for dune fixation and afforestation. The Calligonum L. has a series of advantages, such as drought resistance, high temperature resistance, low soil fertility tolerance, wind erosion and sand burial resistance, easy propagation, strong adaptability, rapid growth, special adaptability to drought and shifting sand. The species distribution, propagation technique, chemical components, biological characteristics of stress resistance and ecological characteristics of the Calligonum L. were reviewed, so as to provide foundation for the study of Calligonum L. on desertification control, stress resistance mechanism and development of their genetic resources.

Sainfoin （Onobrychis viciifolia） is perennial legume forage and is honored as ＂grass queen＂ due to its high nutritive value and good palatability. It has strong resistance to cold and drought and is widely cultivated around the world. Reports on feeding value, diseases and cultivation techniques of sainfoin are common, but literatures on its secondary metabolites are still limited. We systematically reviewed the differences in content and structure of condensed tannins from sainfoin with different varieties and phenological phases. Furthermore, we elaborated the roles of condensed tannins from sainfoin in ruminant nutrition regulation, with emphasize on its effect on digestion and absorption of nutrients, production performance, occurrence of frothy bloat and gastrointestinal parasites infection in ruminant animals.

Based on the data of high ahitude, ground observation, physical quantity field and radar, the process of a rainstorm and hail weather in July 28 of 2016 in Tongliao city of Inner Mongolia was analyzed. The resuks showed that the rainstorm and hail weather process was jointly influenced by upper trough, low level jet and surface convergence line. Meanwhile, the atmospheric stratification stability, vapor condition and dynamic condition were found as the main formation conditions for the rainstorm and hail weather. Furthermore, the radar products showed an indicative significance on forecast of the rainstorm and hail weather.

Based on the temperature data collected from eight meteorological observational stations in Tongliao region of Inner Mongolia from 1961 to 2015, the temperature variation tendency was characterized by using linear trend analysis, Mann-Kendall mutation test and R/S analysis method. The results showed that in recent 50 years, the average temperature, maximum temperature and minimum temperature showed an increasing trend, and the increase mutation points of the annual average temperature was observed in 1988; the burst phenomenon was observed in variation of annual average temperature, seasonal average temperature, maximum temperature and minimum temperature.

Sheep Production is the main course of animal science major and emphasizes the combination of theoretical knowledge and practical ability. According to the features of this course such as extensive teaching content and strong practicality, and existing problems in teaching of this course such as deficient interest in study and poor practical ability of undergraduates, the teaching reform of Sheep Production is conducted and explored in the following aspects of teaching content, teaching method, and examination system. The teaching effect and quality of Sheep Production is improved by implementing the teaching reform, which lays the teaching foundation for cultivation of high quality talents with professional and technical ability that are able to adapt to the modern sheep production.

The interactive teaching model is designed to promote the interaction between teachers and undergraduates in teaching process and is aimed to cultivate the creative thinking, innovative spirit, and creative ability of undergraduates. The reform of teaching content and innovation of teaching methods and teaching model in interactive teaching model of Mathematical Analysis were discussed with emphasis on cultivation of undergraduates＇ creative ability, innovation of thoughts and ideas in education and establishment of interactive teaching model of Mathematical Analysis in college. The application of this teaching model is helpful for the improvement of teaching quality of Mathematical Analysis and for the enhancement of undergraduates＇ innovative spirit and creative ability.

Laboratory Animal Science is multidisciplinary involving in a wide range of content. How to organize and optimize the teaching content is crucial for the teaching of this course. We introduced the experiences in organization and optimization of teaching content of Laboratory A nimal Science in major of animal pharmacy in College of Animal Science and Technology, Henan University of Science and Technology. We proposed some principles that basic knowledge should serve as core, specialty training goal should serve as criterion, and professional curriculums structure should serve as reference, so as to provide help for the selection of teaching content of Laboratory A nimal Science in related colleges.

Physiology of Domestic Animal is a significant professional basic course for undergraduates majoring in veterinary medicine and animal science. According to the cultivation requirement of application-oriented undergraduates, teaching reform is conducted on the aspects of teaching content, teaching methods, practical teaching based on the teaching experience obtained for years. The objective of the teaching reform is to stimulate undergraduates＇ interest in this course, to promote the undergraduates to participate in classroom teaching, and to improve the undergraduates＇ ability in practical operation, comprehensive application of knowledge, independent thinking, problem analyzing and solving.

The teaching innovation system of swine production experiment was investigated and explored based on the traditional experiment teaching. The content, method and examination of teaching innovation was analyzed and thought, so as to cultivate the undergraduates＇ practical ability and creativity and to improve the teaching quality of swine production experiment.

We introduced the necessity for development of silvapastoral and expounded how to establish the teaching system for specialized talent cultivation for animal science major characterized by silvapastoral in Southwest Forestry University with emphasis on the aim and model of cultivating talents. By combination with disciplines associated with forestry science, the specialized talent cultivation model for animal science major characterized by silvapastoral was primarily established and achieved good effect, which provided references for the reform of talent cultivation model for animal science major in associated forestry university and colleges.

The current situation of traceability system of sheep from pastoral area in Inner Mongolia was investigated, and the difficulties and problems in establishing sheep whole industry chain traceability system in pastoral area were pointed out. Based on the investigation results, corresponding countermeasures and suggestions were proposed, so as to provide references for accelerating the construction of traceability system of sheep from pastoral area, improving brand competitiveness of sheep products from pastoral area, and increasing economic income of herdsmen from sheep breeding.

Canine distemper is a highly contagious infectious disease caused by canine distemper virus （CDV）. Animals in Canidae, Mustelidae and Procyonidae are susceptible to CDV. This disease can be preliminary diagnosed by clinical symptoms and epidemiological features and confirmed diagnosed by etiology examination and serodiagnosis. Currently, due to the lack of effective drugs for cure of canine distemper the prevention of this disease mainly dependents on vaccination. We summarized the clinical diagnosis technique of canine distemper disease and the research progress on canine distemper vaccine, so as to provide some references for the veterinarians.

Excretory-secretory （ES） products released by Trichinella spiralis play a complex role in regulating host immune system by actions of activation and inhibition. This regulative effect is helpful for Trichinella spiralis to escape from the immune response and then to infect the host. Meanwhile, the immune regulation mechanism induced by Trichinella spiralis can relieve the symptom of autoimmune disease, allergic and cancer.