Abstract: This study aimed to assess the effect of dietary supplementation of Curcuma longa on production performance, egg quality and serum biochemical indexes of laying hens. A total of 48 healthy Roman brown shell laying hens were chosen and averagely and randomly assigned to one of the two groups： an experimental group fed a special diet of feed supplemented with 1%Curcuma longa powder, and a control group fed basal diet. The experimental period lasted for 48 days. The indexes associated with production performance, egg quality and serum biochemistry of the laying hens from experimental group and control group were determined and statistically evaluated. The results showed that compared with the control group, experimental group had higher feed intake and laying rate（P〈0.05） and lower feed-egg ratio（P〉0.05）; the egg protein content of experimental group was significantly higher than control group（P〈0.05）, and the cholesterol content per 1 g of egg yolk and egg cholesterol content of experimental group was very significantly lower than control group（P〈0.01）; the experimental group had very significantly higher content of HDL-C than control group（P〈0.01） and had very significantly or significantly lower content of LDL-C, TG and TC than control group（P〈0.05 or P〈0.01）. The results obtained in this study indicated that the dietary supplementation of Curcuma longa showed the ability to reduce egg cholesterol content and to improve lipid metabolism function of laying hens.