Abstract: In the present study, the growth of Pochonia chlamydosporia ARSEF3539 was characterized in the aspect of growth process, morphology, and development and formation of conidia and chlamydospore. The results showed that the growth rate of Pochonia chlamydosporia ARSEF3539 in 0.4 g/L of corn meal agar （CMA） medium was slightly different from that in savary- gilliard glucose agar （SDA） medium; the strain grew faster in SDA medium and its clones were thick and white in flocculent shape; the strain grew slower in CMA medium and its clones were thin in flocculent shape; the hyphae on the two medium were radial, thick and developed; conidiophores were single or verticillately distributed in hyphae; there were 4-5 branches in each conidiophore and the conidia aggregately grew on top of each branch; the spore was transparent and was oval or round in shape. Chlamydospore with thick wall was formed in the aged hyphae and each was multicellular in a brick lattice shape.