Abstract: In order to evaluate the application effect of a commercially available fulvic acid nutrient solution with trade name of Huangfutian in typical steppe of Inner Mongolia and to assess the correlation of fertilizer with yield, quality and soil of natural grassland, a fertilization experiment was designed and conducted in Handahan Village, Dalinur Town, Hexigten Banner of Inner Mongolia. Three control groups and seven treatments were set in this study. The results of dual factors （fertilizing concentration and fertilizing frequency） experiment showed that the highest average increase of forage yield （2 266.5 kg/hm2） was observed in the treatment of single application of fulvic acid nutrient solution with concentration of 150 kg/hm2 in August. This combination of experimental factors had the highest yield increase benefit of fertilizing in all of the treatments in 2016.