Abstract: The aim of the present study was to characterize the antimicrobial resistance of Enterococcus faecalis（E. faecalis）isolates from swine in Henan Province. A total of 300 batches of samples were collected from scaled pig farms; the isolation,culture, and purification of E. faecalis strains were performed by using conventional methods; the isolates were subsequently identified to species level by using automatic bacterial identification/antimicrobial susceptibility test system and PCR assay;micro-broth dilution method was used to evaluate the susceptibility of the isolates to antimicrobial agents. The results showed that a total of 154 strains were identified as E. faecalis and the overall isolation rate was 51.33%; high resistance rates were detected for cefoxitin, clindamycin, tiamulin, ceftiofur, sulfisoxazole, erythromycin and tilmicosin（all above 90%）; isolates also exhibited resistance to doxycycline, florfenicol, penicillin and compound sulfamethoxazole, with resistance rates ranging from 60% to 90%.It is indicated that the antimicrobial resistance status of E. faecalis isolates from swine in Henan Province is serious.