Abstract: In order to effectively solve the problems that blood smear slides and necessary reagents and items used in staining process encroach on the experimental platform space and wasted dyeing solution pollutes the environment, a staining scaffold used in blood smear preparation was developed. The staining scaffold is mainly composed of supporting frame, a number of partitions as well as the wasted liquid tank, staining platform and placing platform successively set from top to bottom of the staining scaffold.The slides smeared with blood sample are put evenly in the staining platforms and subsequently stained; after staining, the partitions are moved up to the placing platform and the staining and drying steps can be performed simultaneously; the remaining liquid produced in the process of smear drying flows to the wasted liquid tank and cannot drip directly. By using the developed staining scaffold, the preparation quality of the blood smear is improved, the duration for drying step is shortened, the centralized collection and treatment of the wasted liquid is beneficial for the biological safety, and the experimental platform space is saved.