Abstract: This study was aimed at evaluating the effects of dietary supplementation of yeast culture on productive performance and economic benefits of beef cattle. A total of 150 Simmental crossbred cattle were randomly assigned to one of two groups such that control group（n=75） fed the TMR diet prepared in the experimental cattle farm and experimental group（n=75） fed the TMR diet supplemented with 0.5% of yeast culture. The experimental period lasted for 90 days. The results revealed that the daily gain and economic benefits were found increased by 9.68%（P〈0.05） and 9.09% in the experimental group compared to the control group respectively, and the ratio of feed to gain was found decreased by 1.79%（P〈0.05） in the experimental group compared to the control group. The results obtained in this study suggest that supplementing the basil diet with yeast culture improves the productive performance and economic benefits of beef cattle.