Abstract: In order to determine the seroprevalence of rabies virus among dogs in Wenzhou of Zhejiang Province, plasma samples of 351 dogs were tested forIgG antibodies against rabies virus by ELISA. The results revealed that rabies virus IgG antibodies were detected in 163（46.44%） of the 351 dogs; male dogs had slightly higher antibodies positive rate compared to female ones（P〉0.05）, and the seroprevalence was extremely significantly higher in neutered dogs than intact dogs（P〈0.01）; extremely significantly higher seroprevalence was observed in adult dogs compared to young（P〈0.01） and geriatric dogs（P〈0.01）; the seroprevalence in household dogs was extremely significantly higher than dogs in training field（P〈0.01） and stray dogs（P〈0.01）. The combined data suggest that the rabies virus prevails in dogs at a moderate level in Wenzhou, the management of dogs in training field and stray dogs should be enhanced, and the immunization coverage against rabies virus for dogs should be extended, so as to control the spread of rabies in this region.