Abstract: The aim of the present study was to give an overview of the positive role of electric field on alfalfa. The biological effects of electric field on alfalfa were expounded with focus on high voltage electric field treatment for seeds, plants and callus of alfalfa. We elaborated the influence of electric field on seed germination rate, seed germination energy, water content of seedlings, growth and development of seedlings, cold and drought resistance, and callus rate of alfalfa, in hoping to provide scientific basis for further research concerning biological effects of electric field on alfalfa.