Abstract: A case of transport stress syndrome occurred in a beef cattle farm in Jiangxi Province was recorded. A total of 67 beef cattle around one-year-old were introduced from Jilin province; at the 5th day of introduction, 38 of （57%） the beef cattle were observed to be diseased in succession and 8 of them were died with a mortality of 12%; the general clinical signs of the diseased beef cattle comprised of dyspnea and asthma; some of the diseased beef cattle had severe diarrhea and joint swelling; the necropsy results revealed that the diseased beef cattle were subjected to cellulosic pleural pneumonia, and several of them had purulent and tyroid necrotic foci in lungs; there were ulcer foci in abomasum and small intestine; two poles stained bacillus were observed in microscopic examination and dewdrop -shaped small colonies were obtained in blood agar plate after incubation of clinical samples. Collectively, it was indicated that the pasteurella infection is responsible for the occurrence of the disease. We report the incidence and diagnosis of a case of transport stress syndrome in beef cattle, in hoping to provide references for the clinical practice.