Abstract: In order to optimize the β-cyclodextrin （β-CD） inclusion process of volatile oil from Radix Angelicae Pubescentis andimprove the efficacy of its preparation, a three-factor and three-level orthogonal test was designed to optimize the key factorsaffecting the ultrasonic inclusion process, with inclusion rate and inclusion compound yield as indicators. The results showed thatthe optimal inclusion process was as follows： ultrasonic time of 30 min, volatile oil/β-CD ratio of 1：10 （mL：g） and watedl3-CDratio of 11：1 （mL：g）. This study will provide technical support for improving the utilization efficacy of the active ingredients ofRadix Angelicae Pubescentis.