Abstract: In the present study,the ACTA1 gene of Yanbian Yellow cattle was cloned by RT-PCR assay and subsequently bioinformatically analyzed with bioinformatics analysis software.The results showed that the CDS of ACTA1 gene was composed of 1 134 nucleotides which encoded 377 amino acid residues;topological prediction revealed that the protein encoded by ACTA1 gene had four casein kinaseⅡphosphorylation sites,eight N-myristoylation sites,and one ACTIN conserved domain.Results of this study provide references for understanding the mechanism underling the regulative effect of ACTA1 gene on meat quality in bovine.