Abstract: The aim of this study is to determine the differences of meat quality and blood biochemical parameters between crossbreed yellow cattle and local breed yellow cattle in high altitude areas. For blood free amino acid concentrations, Met, Ile, Phe,Lys and Tyr of the local breed yellow cattle were significantly higher than those of cross breed yellow cattle respectively （P〈0.05）,Try, Glu, Ser, Gly, Ala, Cys, Val, Leu, His and Arg were also higher than cross breed yellow cattle （P〉0.05）. Thr, Glu and Proconcentration of the local breed yellow cattle were lower than cross breed yellow cattle respectively （P〉0.05）. Total amino acid,essential amino acid and non-essential amino acid were higher in blood samples of local breed yellow cattle compared with crossbreed yellow cattle （P〉0.05）. Total cholesterol, urea nitrogen and total anti-oxidant activity were higher in local breed yellowcattle compared with cross breed yellow cattle（P〈0.05 ）. Low density lipoprotein was higher in local breed yellow cattle than crossbreed yellow cattle （P〈O.01）. No differences were found in C3, triglyceride, glucose, IgA, IgG, IgM, MDA, GSH-px andlipoprotein cholesterol between local breed yellow cattle and cross breed yellow cattle respectively （P〉0.05）. For meat quality, nodifferences were found in marbling score, shearing force, cooking lose and lion eye area between local breed yellow cattle andcross breed yellow cattle（P〉0.05 ）.