Abstract: In this study,the shredded leather was used as raw material to prepare pet dog chew.After alkali liquor soaking,heating,pulping as well as adding auxiliary material and transglutaminase,the slurry was prepared.Subsequently,the slurry was subjected to formation and dehydration,and then the recombined cowhide for preparation of pet dog chew was obtained.In order to screen the optimum preparation technology of recombined cowhide,the effects of solid-liquid ratio,soybean protein isolate addition and transglutaminase addition on property of recombined cowhide were assessed,and the sensory indexes and mechanical characteristics of the recombined cowhide were determined.The optimum conditions were determined as followed:20 g of shredded leather were mixed with 120 mL of 0.1 mol/L NaOH solution and heated in 100℃water bath for 1.5 h;the mixture was added with 0.4 g of glycerinum,0.2 g of propylene glycol and 0.8 g of starch,after homogenate,the slurry was obtained;the ratio of slurry and water was 3∶1,and the addition of soybean protein isolate and transglutaminase was 10%and 0.5%,respectively.The tensile strength and hardness of the recombined cowhide were moderate,the solubility and transmission were good,and the color was close to the original cowhide.