Abstract: In an attempt to improve the developmental rate of bovine in vitro fertilized sexed embryo, the embryos cultured in vitro for 8 h were used as material to assess the effect of different cumulus cell removal methods （mechanical removal method, enzyme removal method, oscillation removal method） on efficiency of in vitro fertilization （IVF） of sex-sorted semen. The results revealed that the cleavage rates of the fertilized eggs treated with the three cumulus cell removal modes after 48 h in vitro culture were （58.8±2.8）% ,（58.5±5.7）% and （69.9±3.4）%, respectively, and the blastocyst rates after 192 h in vitro culture were （26.7±2.7）%,（26.9±3.9）% and （35.8±4.1）%, respectively. It was demonstrated that the oscillation removal method had significantly better performance compared to the other two cumulus cell removal methods （P〈0.05）. In conclusion, when the sex-sorted semen is used to produce bovine in vitro fertilized embryo, the removal of cumulus cell by oscillation method from the embryos after 8 h in vitro culture can significantly improve the developmental rate of bovine in vitro fertilized sexed embryo.