Abstract: To evaluate the effects of different mediums on growth characteristics and oil accumulation of microalgae FACHB-1067, three different mediums, including BG11, BG11-N and SE, were used to culture microalgae FACHB-1067 under the conditions of 20 ℃, 2 084 lx light and 16 h∶16 h light-dark cycle. The bioaccumulation, specific growth rate, oil content and oil yield of microalgae FACHB-1067 in different mediums were investigated and compared by statistical method. The results showed that the specific growth rate of microalgae FACHB-1067 in SE medium was the fastest, which was 1.052/d; the ranking of the biomass accumulation in three mediums was SE （2.532 g/L） 〉BG11 （2.032 g/L） 〉BG11-N （1.916 g/L）; the oil content and yield in BG11 medium were 28.64% and 1.628 mg/（L·d）, respectively, which were significantly higher than those in BG11-N and SE medium （P〈0.05）. In summary, SE medium showed better performance in specific growth rate and biomass accumulation, while BG11 medium showed better performance in oil content and oil yield.