Abstract: In order to promote the harmless treatment and resourceful utilization of manure in livestock and poultry farm, a field experiment was conducted to assess the effects of substitution of chemical fertilizers with digested pig biogas slurry on growth traits, yield and quality of indica hybrid rice. A total of ten experimental groups with different application amount of biogas slurry and containing varied nitrogen content, including N20% （N2 group）, N40% （N4 group）, N60% （N6 group）, N80% （N8 group）, N100% （N10 group）, N120% （N12 group）, N140% （N14 group）, N160% （16 group）, N180% （N18 group） and N200% （N20 group）, were set in this study. Furthermore, a blank control group （CK） without application of nitrogen fertilizer and a conventional fertilizer control group （CF） were also included. The results showed that the CF and the biogas slurry application groups （N2-N20） had better performance in growth traits, yield and quality of indica hybrid rice compared to CK; while the plant height of the N2 and N4 groups were both significantly reduced compared with the CF （P〈0.05）, no significant differences were observed between the other experimental groups and the CF （P〉0.05）; there were no significant differences in effective panicle and tiller number between the biogas slurry application groups （N2-N20） and the CF （P〉0.05）;while the yield of brown rice in biogas slurry application groups （N2-N20） exhibited an increasing tendency compared to the CF, no significant differences were found （P〉0.05）. The straw yield of the biogas slurry application groups （N2-N20） generally displayed a linear increase compared to the CF, and a significant difference was observed in the N18 group （P〈0.05）. Additionally, except for N2, N10 and N12 groups, the other biogas slurry application groups had significantly higher thousand seeds weight compared to the CF （P〈0.05）; expectedly, the protein content of brown rice of the biogas slurry application groups （N2-N20） showed a generally increasing trend, and significant differences were found both in the N18 and N20 groups （P〈0.05）. Although the amylose content of brown rice of the biogas slurry application groups （N2-N20） displayed a certain decreasing trend compared to the CF, no significant differences were observed （P〉0.05）. Collectively, the obtained results preliminarily indicated that the fertilizer efficiency of N120% digested pig biogas slurry was basically similar to that of the conventional urea fertilizer, and the indica hybrid rice still grew well when the urea was substituted with the excessive application amount of biogas slurry up to N200%. Furthermore, the increase of biogas slurry application amount improved the nutritional value of grains of indica hybrid rice.