Abstract: An experiment was conducted using a completely randomized design 2×5 factorial arrangements to investigate the assess the influence of different sources and levels of dietary manganese on growth performance and mineral tissue deposition of broilers.A total of 540 healthy Avian broilers were assigned randomly to one of ten dietary treatments of 3 replicate cages each with 18 chicks per cage,and fed a basal corn-soybean meal diet containing 0,45,90,130,180 mg of Mn/kg from a compound Mn-amino acid(Mn-AA)preparation or the basal diet supplemented with 0,45,90,130,180 mg of Mn/kg from MnSO4·H2O.The feeding experiment lasted for 42 days.At 35 days of age,the growth performance indexes of the broilers were determined,and the feed to gain ratio was calculated;at 42 days of age,the contents of copper,iron,zinc in tibia,liver and kidney and the levels of ash,calcium and phosphorus in tibia were evaluated.The results showed that when the dietary manganese supplementation levels ranged from 45 to 130 mg/kg,there were significant differences in 35-day-old weight and feed intake between Mn-AA or MnSO4·H2O supplemented treatments and Mn-unsupplemented treatments(P≤0.05),and the different dietary levels of Mn had a statistically significant effect on average weight and feed intake(P<0.01);no significant differences in 35-day-old weight,feed intake and feed to gain ratio were observed in the broilers fed the diet supplemented with Mn from different sources(P >0.05).There were no significant effects of different sources or levels of dietary manganese and their interactions on the contents of copper,iron,zinc in tibia,liver and kidney and the levels of ash,calcium and phosphorus in tibia of broilers 42 days of age(P >0.05).Our results demonstrate that dietary manganese supplementation levels have a very significant effect on growth performance of broilers(P<0.01),different sources of dietary manganese express no significant effect on growth performance of broilers(P >0.05),and the sources or levels of dietary manganese and their interactions exhibit no significant effect on mineral tissue deposition of broilers(P >0.05).