Abstract: The aim of this study was to establish a high-performance liquid chromatography (HPLC) method for simultaneous determination of aflatoxin B1,B2,G1 and G2 in feed.The sample was extracted with methanol solution,purified by immune-affinity column,separated by C18 reverse phase column,derivatized by photochemical derivatizer,and analyzed by fluorescence detector.The results showed that the four tested aflatoxins showed a good linear relationship in the range of 0.5-20 ng/mL,and the recovery was 71.6%-89.0%.The established HPLC method was used to detect the total amount of aflatoxin in five kinds of feed samples.The detection rate ranged from 74.0% to 86.7%,and the detected value of aflatoxin ranged from 0.5 to 4.3 μg/kg.In conclusion,the established HPLC method has high precision and good repeatability,providing an effective analytical method for monitoring the level of aflatoxin in feed.