Abstract: The large-scale breeding farms of beef cattle in Yichun area have developed rapidly,and the prevalence of tuberculosis in beef cattle population may cause huge economic losses.In order to understand the infection status of tuberculosis in a large-scale beef cattle farm in Yichun area,more than 300 beef cattle in this farm were randomly sampled and tested by γ-interferon ELISA.The results showed that the positive rate of the randomly selected beef cattle samples was 3.33%.Higher positive rate (6.67%) of tuberculosis was both observed in the beef cattle population older than 12 months and the population tested in spring.The data show that the vaccine efficacy against tuberculosis in this cattle farm is poor,and effective prevention and control measures are urgently needed.